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I started another couple of jars today


dixonpete

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I was in a bit of a rush and didn't finesse the job as well as I would have liked. I ended up using too much stool in one jar and not enough on the other. The overweight one might tear through the threads and sink into the water, and the light one might not have enough stool to generate many larvae. This experience tells me there's some technique to this, and I'm thinking going forward I'll tape down the threads to the outside of jars before placing the stool on the filter and then pipette water into the base of the jars.

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So not great, but if things don't work out this incubation I have time to do another, this time exercising more care.

Also, I think I'll take a sample each day under the scope to see when larvae start appearing. It's supposed to be 5 days at high room temps and 9–10 days and normal room temperature. Let's find out for sure. 

I'm doing fine. Still staying away from peanuts. No gut adversity to report.

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dixonpete

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Day 2, the day after incubation, I found 1 inactive larvae after sampling both jars. By sampling I mean inspecting about 15 mls of water extracted from each jar.

Day 3, nothing.

Day 4, I saw one larva in each jar.

Day 5, I estimate there were 15–20 larvae in each jar. Enough to inoculate.

So I guess that's the answer. 5 days. Waiting longer would mean less work as there would be less time scanning the petri dish.

I live near Detroit and this is April. Average temperature in my home has ranged btw 19 and 23C.

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