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Rating of 4 jars for this incubation round


dixonpete

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Yesterday I inoculated myself with 25 Necator americanus larvae from the small red jar. There were so many larvae, all I had to do was find a couple of drops that had the number of larvae I was looking for and pipette them onto the bandage. No messing with sucking up individual larvae and placing them in a tube. Much quicker, and why having a large crop is a good thing. Why choose the number 25? I didn't feel like bothering with more frequent inoculations, and 25 was as high as I was prepared to go. My next inoculation will be in 4 months and 15 days. It takes a month for the larvae to start working (and become adults) and I've lost before benefits at six months+. 4m/15d leaves plenty of margin. That date will be the 14th of January 2025. Note that date is inoculation, not incubation, which takes about a week+.

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This time I used a roll of Athletic Wrap Tape / Self Adhesive Elastic Bandage to keep the bandage attached to my arm while sleeping. It worked well.

I used 4 jars for the incubation this time around. Two large and two small. There was a very wide variation in the number of larvae the jars produced even though they were prepared the same way.

Small red - A+ - 10 to 15 active larvae per drop. An ideal result

Small green - F - I didn't see any larvae in any drops from this jar

Big red - B -  Enough for inoculation but it would mean work collecting 

Big Green - E -  Very few larvae, perhaps not enough for inoculation

Again, I don't get why there is so much variation. The set-up is simple. Add a small amount of water to a sealable jar, stuff a coffee filter lightly smeared with stool containing hookworm eggs. Make sure the filter paper hits the water so the larvae can climb down, and wait about 7 days. It doesn't get much easier than that. Going forward, I'll stick with four small jars. Maybe some day I'll figure out why all this is. At least next incubation, I won't have to worry about the heat being a factor.

Athletic Wrap.PNG

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dixonpete

Posted

Seven days in the mark from the inoculation is becoming much less noticeable. I expect by ten days all that will be left will be some slight redness.

I had worried that the pressure bandage would crush the larvae but apparently they minded it not at all. The wrap made keeping the bandage in place much easier. Highly recommended. 

dixonpete

Posted

I should have added that before the stool is smeared on the coffee filter, it must be thoroughly moistened with mineral water. This is an important step.

There have been reports on the Helminthic Therapy Incubation Forum that without doing that, the larvae can't get out of the stool and down to the water at the bottom of the jar.

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