Pete Dixon

Yesterday I inoculated myself with 25 Necator americanus larvae from the small red jar. There were so many larvae, all I had to do was find a couple of drops that had the number of larvae I was looking for and pipette them onto the bandage. No messing with sucking up individual larvae and placing them in a tube. Much quicker, and why having a large crop is a good thing. Why choose the number 25? I didn't feel like bothering with more frequent inoculations, and 25 was as high as I was prepared to go. My next inoculation will be in 4 months and 15 days. It takes a month for the larvae to start working (and become adults) and I've lost before benefits at six months+. 4m/15d leaves plenty of margin. That date will be the 14th of January 2025. Note that date is inoculation, not incubation, which takes about a week+.

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This time I used a roll of Athletic Wrap Tape / Self Adhesive Elastic Bandage to keep the bandage attached to my arm while sleeping. It worked well.

I used 4 jars for the incubation this time around. Two large and two small. There was a very wide variation in the number of larvae the jars produced even though they were prepared the same way.

Small red - A+ - 10 to 15 active larvae per drop. An ideal result

Small green - F - I didn't see any larvae in any drops from this jar

Big red - B -  Enough for inoculation but it would mean work collecting 

Big Green - E -  Very few larvae, perhaps not enough for inoculation

Again, I don't get why there is so much variation. The set-up is simple. Add a small amount of water to a sealable jar, stuff a coffee filter lightly smeared with stool containing hookworm eggs. Make sure the filter paper hits the water so the larvae can climb down, and wait about 7 days. It doesn't get much easier than that. Going forward, I'll stick with four small jars. Maybe some day I'll figure out why all this is. At least next incubation, I won't have to worry about the heat being a factor.