Pete Dixon

I did another test incubation that turned out to be successful. Though it was a month early, I didn't see any harm in inoculating early with 25 larvae.

Celiac.com Sponsor (A13):
This time I used 5 jars. Three had zero larvae, one had an ok yield, and the last had good/very good population. The included pic is from that last jar. With that kind of density, collecting the needed number of larvae is quick work. 

This experience reinforces to me the necessity of using multiple jars. Five seems to be the right number, again with explanation that some portions of the stool being sampled for the jars simply don't contain eggs.