Jump to content
This site uses cookies. Continued use is acceptance of our Terms of Use and Privacy Policy. More Info... ×
  • Welcome to Celiac.com!

    You have found your celiac tribe! Join us and ask questions in our forum, share your story, and connect with others.




  • Celiac.com Sponsor (A1):



    Celiac.com Sponsor (A1-M):


  • Get Celiac.com Updates:
    Support Our Content
    eNewsletter
    Donate
  • Record is Archived

    This article is now archived and is closed to further replies.

    Jefferson Adams
    Jefferson Adams

    Autophagy Protein LC3 Tied to Accumulation of Dendritic Cells in Celiac Disease

    Reviewed and edited by a celiac disease expert.

    Celiac.com 04/28/2014 - Accumulation of dendritic cells (DCs) in duodenal mucosa is associated with celiac disease. Autophagy protein LC3 has recently been implicated in autoantigen formation. However, its role in celiac disease remains unknown.

    Image: Wikimedia Commons.A team of researchers recently set out to examine role of autophagic protein LC3 expressed by activated DCs in celiac disease. The research team included P. Rajaguru, K. Vaiphei, B. Saikia, and R. Kochhar, with the Department of Histopathology, Post Graduate Institute of Medical Education and Research in Chandigarh, India.

    Celiac.com Sponsor (A12):
    The team analyzed thirty celiac disease patients at initial presentation and after 6 months of gluten-free diet (GFD). They examined duodenal biopsies for histological changes and CD11c, CD86, and MAP1LC3A expressions by double immunohistochemistry (IHC).

    They used Masson's trichrome (MT) staining to determine basement membrane (BM) thickness and Oil Red O (ORO) staining for mucosal lipid deposit. They also conducted polymerase chain reaction (PCR) for the HLA-DQ system. For statistical analysis, they used paired and unpaired t test, chi-square test, Fisher's exact test, and McNemar-Bowker test. A P-value

    They found HLA-DQ2 and HLA-DQ8 alleles in all study subjects. They also observed thicker BM in 63% of subjects, while 73% of subjects showed ORO-positive lipid in surface lining epithelium.

    Pre-treatment biopsies showed a higher number DCs expressing LC3, which dropped significantly in follow-up biopsies, and also showed significant reduction in BM thickness and ORO.

    These results show that histological improvement in duodenal biopsies is connected with less activated DCs expressing autophagic protein, which likely play important role in the development of celiac disease and other autoimmune disorders.

    Source:

    • Open Original Shared Link


    User Feedback

    Recommended Comments

    There are no comments to display.



    Guest
    This is now closed for further comments

  • Get Celiac.com Updates:
    Support Celiac.com:
    Donate
  • About Me

    Jefferson Adams

    Jefferson Adams is Celiac.com's senior writer and Digital Content Director. He earned his B.A. and M.F.A. at Arizona State University. His articles, essays, poems, stories and book reviews have appeared in numerous magazines, journals, and websites, including North American Project, Antioch Review, Caliban, Mississippi Review, Slate, and more. He is the author of more than 2,500 articles on celiac disease. His university coursework includes studies in science, scientific methodology, biology, anatomy, physiology, medicine, logic, and advanced research. He previously devised health and medical content for Colgate, Dove, Pfizer, Sharecare, Walgreens, and more. Jefferson has spoken about celiac disease to the media, including an appearance on the KQED radio show Forum, and is the editor of numerous books, including "Cereal Killers" by Scott Adams and Ron Hoggan, Ed.D.

    >VIEW ALL ARTICLES BY JEFFERSON ADAMS

     


  • Celiac.com Sponsor (A17):
    Celiac.com Sponsor (A17):





    Celiac.com Sponsors (A17-M):




  • Related Articles

    Jefferson Adams
    A team of researchers with the Department of Medicine at the University Erlangen-Nuernberg in Germany recently set out to examine the role of the innate immune system in celiac disease. The team included Maryam Rakhimova, Birgit Esslinger, Anja Schulze-Krebs, Eckhart G. Hahn, Detlef Schuppan and Walburga Dieterich.
    The researchers matured dendritic cells taken from venous blood of patients with both active and with treated celiac disease, along with DQ2–DQ8-positive or negative control subjects.  They treated the dendritic cells with a peptic–tryptic digest of gliadin (500 μg/ml)
    and assessed activation by means of fluorescent-activated cell sorting analysis, cytokine secretion, and the cells' ability to trigger T cell proliferation.
    The team noted that gliadin up-reg...


  • Recent Activity

    1. - Scott Adams replied to Vozzyv's topic in Celiac Disease Pre-Diagnosis, Testing & Symptoms
      5

      Weird Symptoms

    2. - Jeff Platt replied to Vozzyv's topic in Celiac Disease Pre-Diagnosis, Testing & Symptoms
      5

      Weird Symptoms

    3. - cristiana replied to Vozzyv's topic in Celiac Disease Pre-Diagnosis, Testing & Symptoms
      5

      Weird Symptoms

    4. - Scott Adams replied to More2Learn's topic in Celiac Disease Pre-Diagnosis, Testing & Symptoms
      1

      Celiac Maybe a Possibility?

    5. - More2Learn posted a topic in Celiac Disease Pre-Diagnosis, Testing & Symptoms
      1

      Celiac Maybe a Possibility?


  • Celiac.com Sponsor (A19):



  • Member Statistics

    • Total Members
      126,106
    • Most Online (within 30 mins)
      7,748

    GFBeachbum
    Newest Member
    GFBeachbum
    Joined

  • Celiac.com Sponsor (A20):


  • Forum Statistics

    • Total Topics
      120.9k
    • Total Posts
      69.2k

  • Celiac.com Sponsor (A22):





  • Celiac.com Sponsor (A21):



  • Popular Now

    • Vozzyv
      5
    • Kathleen JJ
    • Captain173
      10
    • jjiillee
      7
    • Kristina12
      7
  • Popular Articles

    • Scott Adams
    • Scott Adams
    • Scott Adams
    • Scott Adams
    • Scott Adams
  • Upcoming Events

×
×
  • Create New...