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    Researchers Use CRISPR/Cas9 to Produce Low Gluten Wheat Strains

    Reviewed and edited by a celiac disease expert.

    For the first time, researchers have used CRISPR/Cas9 technology to produce low-gluten, non-transgenic wheat.

    Researchers Use CRISPR/Cas9 to Produce Low Gluten Wheat Strains - For the first time, researchers have used CRISPR/Cas9 technology to produce low-gluten, non-transgenic wheat. Photo: CC--Sam Beebe
    Caption: For the first time, researchers have used CRISPR/Cas9 technology to produce low-gluten, non-transgenic wheat. Photo: CC--Sam Beebe

    Celiac.com 10/25/2017 - For people with celiac disease, eating gluten proteins from wheat, barley, and rye triggers an auto-immune response, and the accompanying physical symptoms.

    A team of researchers recently set out to engineer low-gluten wheat strains that also have low-reactivity for people with celiac disease. To meet their goals, the team designed two sgRNAs to target a conserved region adjacent to the coding sequence for the 33-mer in the -gliadin genes. They then sought to evaluate the results.

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    The research team included Susana Sánchez-León, Javier Gil-Humanes, Carmen V. Ozuna, María J. Giménez, Carolina Sousa, Daniel F. Voytas, and Francisco Barro. They are variously affiliated with the Departamento de Mejora Genética Vegetal, Instituto de Agricultura Sostenible (IAS-CSIC), Córdoba, Spain; the Department of Genetics, Cell Biology, and Development, Center for Genome Engineering at the University of Minnesota in Minneapolis, MN, USA; and with the Facultad de Farmacia, Departamento de Microbiología y Parasitología, Universidad de Sevilla, Spain.

    The gliadin gene family of wheat includes four highly reactive peptides, with the 33-mer peptide being the main culprit in celiac patients. In all, the team generated twenty-one mutant lines, all of which showed strong reduction gliadin proteins.

    Of the 45 different genes identified in wild type gliadin, the team mutated up to 35 different genes in one of the lines to achieve an 85% reduction in immunoreactivity. They then identified the transgene-free lines, and found no off-target mutations in any of the potential targets.

    So, what does this all mean in English? Well, basically the low-gluten, transgene-free wheat lines that the team describes here could be used to produce low-gluten foods, as well as serving as source material to introduce the low-gluten, low-reactivity traits into selected wheat varieties.

    Basically, the technology could be used to create low-gluten wheat varieties with low immunoreactivity. Now, most folks with celiac disease, especially those with higher gluten sensitivity, would likely need more than and 85% reduction in immunoreactivity to see any real benefit. However, this study provides an interesting glimpse at how science might help researchers to create wheat strains that are safe for people with celiac disease.

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    sunny2012

    Genetically altering plants has serious risks.

    CRISPR-Edited Cells Linked to Cancer Risk in 2 Studies
    "In the studies, published in Nature Medicine, scientists found that cells whose genomes are successfully edited by CRISPR-Cas9 have the potential to seed tumors inside a patient. That could make some CRISPR’d cells ticking time bombs, according to researchers from Sweden’s Karolinska Institute and, separately, Novartis."
    https://www.scientificamerican.com/article/crispr-edited-cells-linked-to-cancer-risk-in-2-studies/

    Chromothripsis as an on-target consequence of CRISPR–Cas9 genome editing
    “Here, using model cells and single-cell whole-genome sequencing, as well as by editing at a clinically relevant locus in clinically relevant cells, we show that CRISPR–Cas9 editing generates structural defects of the nucleus, micronuclei and chromosome bridges, which initiate a mutational process called chromothripsis. Chromothripsis is extensive chromosome rearrangement restricted to one or a few chromosomes that can cause human congenital disease and cancer.”

    "These results demonstrate that chromothripsis is a previously unappreciated on-target consequence of CRISPR–Cas9-generated DSBs. As genome editing is implemented in the clinic, the potential for extensive chromosomal rearrangements should be considered and monitored."

    https://www.nature.com/articles/s41588-021-00838-7

    Unintended Genomic Outcomes in Current and Next Generation GM Techniques: A Systematic Review
    https://www.mdpi.com/2223-7747/11/21/2997

    Gene editing found to cause chaos in the genome of tomatoes
    https://www.gmwatch.org/en/106-news/latest-news/20239-gene-editing-found-to-cause-chaos-in-the-genome-of-tomatoes

    A CRISPR-induced DNA break can trigger crossover, chromosomal loss and chromothripsis-like rearrangements
    https://www.biorxiv.org/content/10.1101/2023.05.22.541757v1

    CRISPRthripsis in plants

    “The recent findings shed new light on the alleged ‘precision’ of gene scissors: although the new technology can be used to target and cut precise locations in the genome, the consequences of ‘cutting’ the genome are to some extent unpredictable and uncontrollable. Plants obtained from new genetic engineering (New GE) cannot, therefore, be regarded as safe per se, and need to be thoroughly investigated for risks.”

    https://www.testbiotech.org/en/printpdf/3077

     

     

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    Jefferson Adams is Celiac.com's senior writer and Digital Content Director. He earned his B.A. and M.F.A. at Arizona State University. His articles, essays, poems, stories and book reviews have appeared in numerous magazines, journals, and websites, including North American Project, Antioch Review, Caliban, Mississippi Review, Slate, and more. He is the author of more than 2,500 articles on celiac disease. His university coursework includes studies in science, scientific methodology, biology, anatomy, physiology, medicine, logic, and advanced research. He previously devised health and medical content for Colgate, Dove, Pfizer, Sharecare, Walgreens, and more. Jefferson has spoken about celiac disease to the media, including an appearance on the KQED radio show Forum, and is the editor of numerous books, including "Cereal Killers" by Scott Adams and Ron Hoggan, Ed.D.

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